Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion

背景：

目前，使用人血源成分仿佛成為間充質(zhì)干細(xì)胞（mesenchymal stromal cell，MSC）擴(kuò)增zui有效zui安全的方法。這篇文獻(xiàn)介紹了不同血漿來(lái)源的人AB血清的特征，以及人AB血清作為MSC擴(kuò)增的補(bǔ)體使用。

方法：

人AB血清的兩種血漿來(lái)源：全血采集后24h的血漿和去冷沉淀血漿（Cryoprecipitate-reduced plasma）。分析人AB血清不同批次的生化性質(zhì)，并且評(píng)估它們儲(chǔ)存不同時(shí)間后（0，3，6，9個(gè)月）對(duì)MSC細(xì)胞生長(zhǎng)的影響。

結(jié)果：

兩種血漿來(lái)源的人AB血清的生化組成和質(zhì)量參數(shù)類似，而且都能有效促進(jìn)MSC生長(zhǎng)。添加了10%人AB血清的培養(yǎng)基和添加10%胎牛血清（fetal bovine serum，FBS）的培養(yǎng)基相比，MSC的doubling times and cumulative population doublings類似，同時(shí)MSC保持了免疫表型，功能特征和細(xì)胞遺傳學(xué)特征。

結(jié)論：

總之，結(jié)果說(shuō)明人AB血清是有效的FBS替代物，而且生產(chǎn)后能至少使用12個(gè)月，也不影響細(xì)胞增殖和質(zhì)量。

Characterization of Human AB Serum for Mesenchymal Stromal Cell Expansion.

Transfus Med Hemother. 2017 Jan;44(1):11-21. doi: 10.1159/000448196. Epub 2016 Nov 2.

Dos Santos VT1,et al.

Abstract

BACKGROUND:

So far, using human blood-derived components appears to be the most efficient and safest approach available for mesenchymal stromal cell (MSC) expansion. In this paper, we report on the characterization of human AB serum (AB HS) produced by using different plasma sources, and its use as an alternative supplement to MSC expansion.

METHODS:

Two plasma sources were used for AB HS production: plasma removed from whole blood after 24 h of collection (PC > 24 h) and plasma, cryoprecipitate reduced (PCryoR). The biochemical profile and quality of the produced AB HS batches were analyzed and their ability to support MSC cell growth after different storage times (0, 3, 6, 9 and 12 months) was evaluated.

RESULTS:

The two plasma sources used showed similar characteristics regarding biochemical constituents and quality parameters and were effective in promoting MSC growth. MSCs cultured in medium supplemented with 10% AB HS presented similar doubling times and cumulative population doublings when compared to the 10% fetal bovine serum(FBS)-supplemented culture while maintaining immunophenotype, functional features, and cytogenetic profile.

CONCLUSION:

Overall, the results indicate that AB HS is an efficient FBS substitute and can be used for at least 12 months after production without impairing cell proliferation and quality.

Free PMC Article

摘要翻譯

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GemCell™人AB血清來(lái)自于美國(guó)FDA認(rèn)證的AB血型健康男性供體。供體AB血漿加抗凝劑經(jīng)去纖維蛋白處理得到AB血清，一致性較高。所有供體的病毒標(biāo)志物檢測(cè)都是陰性。

人AB血清作為牛血清（FBS、FCS）的替代物，不含外源物，適合組織工程、移植和細(xì)胞治療領(lǐng)域的間充質(zhì)干細(xì)胞擴(kuò)增，及其他免疫系統(tǒng)相關(guān)的細(xì)胞培養(yǎng)和反應(yīng)。

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